PCR-DIAGNOSTICATION OF INHERITED DISEASES OF HORSES

Yriy Kurilenko

Abstract: PCR-diagnostication of two inherited diseases of horses: severe combined immunodeficiency and hyperkalemic periodic paralysis was conducted. There were not found carriers of mutations among the tested animals. The parameters of PCR, the optimum primer annealing temperature and the concentration of the reaction mixture for amplification of the desired gene fragment were optimized.

Keywords: arabian horse breed; DNA-PKсs; immunodeficiency; mutation; polymerase chain reaction, mutation HYPP, Ukrainian race breed of horses.

Date published: 2023-02-01