GENETIC STRUCTURE OF HUCUL HORSES USING MICROSATELLITE LOCI OF DNA

ANDREJ SHELYOV, OKSANA MELNYK, ALEKSANDR IGNATENKO, VLADISLAV SPYRYDONOV, SERGEJ MELNYCHUK, KIRILL KOPYLOV

Abstract: The aim of the present work was to conduct a genetic analysis of 38 Hucul horses by microsatellite DNA. In the study
the genetic structure of Hucul horses was investigated using 12 microsatellite markers (AHT04, AHT05, ASB17, ASB23,
CA425, HMS03, HMS06, HMS07, HTG04, HTG06, HTG07, VHL20) recommended by the International Society of Animal
Genetics (ISAG). The results showed that the mean number of allele across the 12 loci was 7.917 and ranged from 6 (ASB17,
HMS07) to 10 (AHT05). The mean value of observed heterozygosity (0.757) was lower than expected (0.784), indicating the
prevalence in the studied group of animals homozygous genotypes. Despite the fact that all analyzed loci were polymorphic,
the most polymorphic locus was VHL20 (0.820). In turn, the locus HMS07 (0.627) had the lowest value of PIC (0.589).
Combined exclusion probability was 99.99%, that allow us to show the efficiency of selected microsatellite loci of DNA for
genetic analysis of Ukrainian population of Hucul horses.

Keywords: genetic analysis; Hucul horses; microsatellites; polymorphism

Date published: 2023-02-01