TESTING GROWTH FACTORS ON THE MORPHO - FUNCTIONAL STATE OF GAMETTES BY IN VITRO STORAGE

Tania Todorova, Iancho Todorov

Abstract: Aim of our studies was to investigate the effect of the addition of growth factors, in particular IGF-I to the culture medium on the development of (IVM / IVF) oocytes using the ECS. The results show that the in vitro development of the embryos is enhanced in presence of high concentration of IGF-I with the addition of oestrus cow serum and granulosa cells. Addition of 10 ng IGF-I ml-1 to the TCM 199 containing 10% oestrus serum had a positive effect on the cleavage of the embryos (P <0.05). There is a correlation between the dose of IGF-I and embryonic development after the medium containing 50 ng IGF-I ml-1 resulted in a lower percentage of hatched blastocysts at day 9, compared with the medium containing 100 ng IGF-I ml-1. These studies lead to the conclusion that the high concentrations of exogenous IGF-I added to the culture medium in the presence of granulosa cells, serum and endogenous IGF-I accelerate the development of the embryos.

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Date published: 2023-01-31