Study of the effect of electrochemically activated aqueous solutions on Clostridium perfringens after storage for different time periods

Teodora P. Popova

Abstract: Studies were carried out to determine the sensitivity of two strains of Clostridium perfringens – ATCC 13124 and terrain (T) isolated from domestic fowl feces to electrochemically activated aqueous solutions (EAAS) anolyte and catholyte obtained with 0.5% NaCl and a combination of 0.5% NaCl and 0.5% Na2CO3. VirkonS disinfectant, administered at a final concentration of 0.5%, was used as a control. Both strains exhibited high sensitivity to the tested anolytes and in the suspensions with a density of 106 cells/ml they died under their influence for a 5 min interval. Their sensitivity to the VirkonS control disinfectant was the same. Only the anolyte of NaCl inactivated C. perfringens T for more than 10 min. The tested catholytes inactivated C. perfringens ATCC after more than 1 hour. The sensitivity of both strains tested to the catholyte of NaCl was the same. C. perfringens T appeared to be more sensitive to catholyte prepared with NaCl and Na2CO3 and died in his presence for 20 min. These antimicrobial properties of the tested EAAS were saved completely for a period of 2 weeks when stored in the dark at room temperature. Storage for a 4 and 8 week interval did not affect the antimicrobial properties of the two tested anolytes, but in the catholytes these properties weakened for this period and single cells of both strains of C. perfringens survived over 90 min under their influence.

Keywords: activated water; anolyte; antibacterial activity; catholyte; Clostridium perfringens; VirkonS

Date published: 2019-09-19