EFFECT OF ANTIOXIDANT SUPPLEMENTS IN A STORED SEMEN FROM RAMS

Iancho Todorov, Tania Todorova

Abstract: We conducted a study investigating the effect of antioxidant supplements Cysteine and Vitamin E on the standard parameters of a ram seminal fluid after storage for 0, 24, 48, 72 and 96 hours at 5ºC after collection and dilution. 30 ejaculates from two rams were examined, diluted with yolk Tris buffer containing vitamin E (1 mM), cysteine (10 mM) and a control group without antioxidant. After dilution the semen was kept in a refrigerator (5oC) and for 4 days the qualitative parameters (motility, viability) were examined at intervals of 24 hours. Experiments were conducted outside the reproductive season. Results show that by the addition of 1 mM vitamin E or 10 mM L-cysteine to the Tris medium, the quality of the chilled semen ram improves.

Keywords: antioxidants; rams; semen

Date published: 2017-05-18