DNA - MICROSATELLITE ANALYSIS OF THE THOROUGHBRED HORSE POPULATION IN BULGARIA. DETERMINING THE NUMBER OF ALLELES AND ALLELE FREQUENCY IN STUDIED LOCI

R. Vlaeva

Abstract: The study includes 157 Thoroughbred horses enrolled either in the Bulgarian Thoroughbred Stud Book or in the Non-Thoroughbred Register of the breed. We examined the observed number of alleles and the allele frequency in 13 loci. The results were analyzed as for the whole population as well for the most prominent lines within the population in our country. For the whole population we determined total of 72 alleles, which number varied from 3 in loci HMS1 and HTG7 to 9 in locus ASB2. Most of the loci were highly polymorphic which defines the high genetic diversity in the population. The number of observed alleles varies considerably among the sire lines. In terms of that the sire lines of Blacklock and Birdcatcher shows similar values for the number of observed alleles – 64. For the lines of Hurry On and Tourbillon the number of observed alleles is respectively: 32 and 36. Analogical are the variations of allele frequencies among the sire lines.

Keywords: allele number; microsatellites; thoroughbred

Date published: 2017-04-18