Influence of addition of Thymus vulgaris L., Origanum vulgare L. and Tribulus terrestris in the ration of some indices of rams semen Review
Natalya Miteva
Abstract: The reproductive ability of male breeding animals is of great importance for increasing productivity and production efficiency in sheep farming. Improving the quantitative and qualitative indicators of semen, especially when applying artificial insemination, is of great importance for production results on a sheep farm. For years, has been studied on the possibility of improving the quality of the obtained seed material and its storage through the use of various additives of biologically active substances of plant origin.
The aim of this study is to review the possibilities for using some bioactive plants in sheep breeding to improve reproductive performance in male animals. Since the number of plants containing biologically active substances is very large, this review pays special attention to medicinal plants that are widespread and freely growing in Bulgaria, with proven therapeutic effects and have been used for many years in traditional medicine. In our opinion, the most promising in this regard are common thyme (Thymus vulgaris L.), oregano (Origanum vulgare L.) and Tribulus terrestris.
Keywords: Origanum vulgare L.; rams; semen; Thymus vulgaris L.; Tribulus terrestris
Citation: Miteva, N. (2025). Influence of addition of Thymus vulgaris L., Origanum vulgare L. and Tribulus terrestris in the ration of some indices of ram’s semen – Review. Bulgarian Journal of Animal Husbandry, 62(3), 46-53.
| References: (click to open/close) | Ahlam, Z., Souraya, S., Bouchra, E. K., Moudou, M. M., Uxia, Y. R., Angel, Q. A. L., Juan, J. B. G., Pedro, G. H. & Abdelaziz, S. (2024). The impact of Origanum Vulgare essential oil supplementation on sperm motility and subpopulation alterations in bulls, dogs, and rabbits, Research in Veterinary Science, 170, 105 - 200.
Alenezy, E. S., Barakat, I. A. H. & Al Musayeib, N. M. (2019). Effect of Wild Marjoram (Origanum vulgare) Plant Extracts on Capacitation of Sheep Spermatozoa in Vitro, Advances in Bioscience and Biotechnology, 10, 86 - 97.
Al-Najar, E. M, Abdullah, A. M, Al-Rubaye, T. A. & Hadi, S. M. (2022). Evaluation of Marjoram Leaves (Oregano vulgare) as Feed Supplement on Quality of Semen in Awasian Pollination Rams. Archives of Razi Institute, 77(5), 1831-1835.
Al-Zubaidy, F. M., Al-Abedy A. F. L. & Al-Malaly, H. M. H. (2013). Effect of Different Levels of Plants (Thymus vulgaris and Ziziphus spina christi) on Semen Diluents for Awassi Ram. Journal of Kerbala University, 11(4), 235 - 238.
Bansleben, A. C., Schellenberg, I., Einax, J. W., Schaefer, K. & Ulrich, D. (2009). Chemometric tools for identification of volatile aroma active compounds in Oregano. Anal Bioanal Chem., 395, 1503 - 1512.
Baranauskiene, R., Venskutonis, P. R., Dewettinck, K. & Verhe, R. (2006). Properties of Oregano (Origanum vulgare L.), citronella (Cymbopogon nardus G.) and marjoram (Majorana hortensis L.) flavors encapsulated into milk protein-based matrices. Food Research International, 39, 413 – 425.
Baricevic, D., Milevoj, L. & Borstnik, J. (2001). Insecticidal effect of Oregano (Origanum vulgare L. ssp. hirtum Letswaart) on the dry bean weevil (Acanthoscelides obtectus Say). Horticultural Science, 7(2), 84 – 88.
Chalchat, J. C. & Pasquier, B. (1998). Morphological and chemical studies of Origanum clones: Origanum vulgare L. ssp. vulgare. Essen. Oil Res., 10, 119 – 125.
Chauhan, R. S. & Nautiyal, M. C. (2007). Seed germination and seed storage behavior of Nardostachys jatamansi DC., an endangered medicinal herb of high altitude Himalaya. Curr. Sci., 92(11), 1620 – 1624.
D’Antuono, L. F., Galleti, G. C. & Bocchini, P. (2000). Variability of essential oil content and composition of Origanum vulgare L. populations from a North Mediterranean area (Liguria Region, Northern Italy). Annals of Botany, 86, 471 – 478.
Dcunha, R., Hussein, R. S., Ananda, H., Kumari, S., Kumar Adiga, S., Kannan, N., Zhao, Y. & Kalthur, G. (2024). Current Insights and Latest Updates in Sperm Motility and Associated Applications in Assisted Reproduction. Reprod Sci., 29(1), 7 – 25.
Dimitrov, M., Georgiev, P. & Vitanov, S. (1987). Use of Tribestan on rams with sexual disorders. Veterinary Science (Sofia), 24, 102 - 108 (BG).
Durmich, Z. & Blache, D. (2012). Bioactive plants and plants products: Effect on animal function, health and welfare, Animal Feed Science and Technologies, 176, 150 – 162.
Fei, H., Guang-qiang, M., Ming, Y., Li, Y., Wei, X. & Ji-Cheng, S. (2017). Chemical composition and antioxidant activities of essential oils from different parts of the oregano. Journal of Zhejiang University Science B (Biomed & Biotechnol), 18(1), 79 – 84.
Gaur, R. D. (1999). Flora of the District Garhwal North western Himalaya (with ehtnobotanical notes). Transmedia, Srinagar Garhwal, India.
Gauthaman, K. & Adaikan, P. G. (2006). Effect of Tribulus terrestris on nicotinamide adenine dinucleotide phosphate-diaphorase activity and androgen receptors in rat brain, Journal of Ethnopharmacology, 96, 127 – 132.
Gauthaman, K. & Ganesan, A. P. (2008). The hormonal effects of Tribulus terrestris and its role in the management of male erectile dysfunction—an evaluation using primates, rabbit and rat. Phytomedicine, 15, 44 – 54.
Gayoso, L., Roxo, M., Cavero, R. Y., Calvo, M. I., Ansorena, D., Astiasaran, I. & Wink, M. (2018). Bioaccessibility and biological activity of Melissa officinalis, Lavandula latifolia and Origanum vulgare extracts: Influence of an in vitro gastrointestinal digestion. Journal of Functional Foods, 44, 146 – 154.
Gumus R, Ercan N, & Imik H. (2017). The Effect of Thyme Essential Oil (Thymus Vulgaris) Added to Quail Diets on Performance, Some Blood Parameters, and the Antioxidative Metabolism of the Serum and Liver Tissues. Brazilian Journal of Poultry Science, 19(2), 297-304. http://dx.doi.org/10.1590/1806- 9061-2016-0403.
He, F. & Hubbell, S. P. (2005). Physical Review Letters, 95, 23 – 46.
Hristov, K., Parvanov, P., Vasilev, N., Fasulkov, I. & Karadaev, M. (2017). Effect of non-hormonal agents Trivestan and Lactina® on the sexual behavior and quality of semen in rams during non-breeding season, Bulgarian Journal of Veterinary Medicine, 20(Suppl. 1), 297 – 301.
Ismail, A. A., Abdel-Khalek, E., Khalil, W. A., Yousif, A. I., Saadeldin, I. M., Abomughaid, M. M. & El-Harairy, M. A. (2020). Effects of mint, thyme, and curcumin extract nanoformulations on the sperm quality, apoptosis, chromatin decondensation, enzyme activity, and oxidative status of cryopreserved goat semen. Cryobiology, 97, 144 – 152.
Kchikich, A., Kirschvink, N., Raes, M., El Otmani, S., Chebli, Y., Bister, J. L., El Amiri, B., Barrijal, S. & Chentouf, M. (2024). Carvacrol and Thymol Enhance the Quality of Beni Arouss Buck Semen Stored at 4 ◦C Thanks to Their Antimicrobial Properties. Veterinary Science, 11, 406.
Khnissi, S., Ben Salem, I., Bejaoui, B., Fattouch, S., Mustapha, S., Haj‐Kacem, R., M'Hamdi, N., Martin, P., Dattena, M. & Lassoued, N. (2024). Antioxidant Capacity of Thyme (Thymus vulgaris) Essential Oil and Its Effect on In Vivo Fertility of Rams Subjected to Testicle Heat Stress. Journal of Animal Physiology and Animal Nutrition, 1 – 12.
Khnissi, S, Maaloul, R, Chalouati, H, Mara, L, Aouadi, D, Adouani, B, Dattena, M, Khemiri, I & Fattouch, S. (2025). Nanotechnologyassisted cryopreservation of ovine semen: evaluation of Thymus vulgaris essential oil as a natural antioxidant. Frontiers in Animal Science, 5, 1479602.
Kistanova, E. (2005). Improvement of reproductive performance of rams by the biological active substances – plant extract and probiotic, Biotechnology in Animal Husbandry, 21(5-6), 69 – 72.
Kostova, I. & Dinchev, D. (2005). Saponins in Tribulus terrestris–chemistry and bioactivity. Phytochemistry Review, 4, 111 – 137.
Leyva-Lopez, N., Gutierrez-Grijalva, E. P., Vazquez-Olivo, G. & Heredia, J. B. (2017). Essential oils of Oregano: Biological activity beyond their antimicrobial properties, Molecules, 22(6), 989.
Mahfoudhi, N., Ksouri, R. & Hamdi, S. (2016). 11-Nanoemulsions as potential delivery systems for bioactive compounds in food systems: preparation, characterization, and applications in food industry. Emulsions, Nanotechnology in the Agri-Food Industry, 3, 365 – 403.
Martindale, W. (1972). The extra pharmacopoeia. 26th Edn.: The Pharmaceutical Press.
Mbongue, F. G. Y., Kamtchouing, P., Essame, O. J. L., Yewah, P. M., Dimo, T. & Lontsi, T. (2005). Effect of the aqueous extract of dry fruits of Piper guineense on the reproductive function of adult male rats. Indian J. Pharmacol., 37, 30 – 32.
Meena, A., Sharma, K., Jain, V., Pal, B., Ajit, K., Singh, U., Singh, R. & Rao, M. (2010). Physicochemical and preliminary phytochemical studies on the fruit of tribulus terrestries linn. Res. J. Sci. Technol., 2(2), 31 – 33.
Mirzadeh, K., Beiranvand, S., Vakili, S. T., Mohammadabadi, T. & Minkazemizadeh, A. (2019). Effect of Different Levels of Tribulus terrestris Powder on quantity and Quality Characteristics of sperm of Arabic Male Lambs. Animal Science Journal (Pajouhesh & Sazandegi), 129, 15 – 24.
National Institutes of Health. (2016). Vitamin B6. U.S. Department of Health & Human Services. https://ods.od.nih.gov/factsheets/VitaminB6-HealthProfessional/.
Panova, D. & Tomova, M. (1970). Screening of Tribulus terrestris L. for phenolic compounds. Farmtsiya, 20, 29 - 31 (BG).
Patel, D. K., Kumar, R., Prasad, S. K. & Hemalatha, S. (2011). Pharmacologically screened aphrodisiac plant - a review of current scientific literature. Asian Pac. J. Trop. Biomed., 1, 131 – 138.
Pour, S. S., Pirestani, A., Alirezai, M. & Shafiyei, K. (2015). The effect of adding different levels of aqueous extract of Tribulus terrestris in the extender on sperm motility Afshari rams at 5°C. Journal of Chemical and Pharmaceutical Research, 7(4), 957 – 959.
Randel, R. D., Chase, C. C. & Wyse, S. J. (1992). Effects of gossypol and cottonseed products on reproduction of mammals. Journal of Animal Science, 70, 1628 – 1638.
Rizk, A. M. (1986). The phyto chemistry of flora of qatar. King Print Of Richmond, Great Britian.
Semerdjieva, I. (2013). Biological and phytochemical investigation of Tribulus terrestris L. in Thracian floristic region (PhD thesis), BAS, Sofia (BG).
Sharangi, A. B. & Guha, S. (2013). Wonders of leafy spices: Medicinal properties ensuring Human Health. Science International, 312 – 317.
Sharaway, S. M., Saleh, N. H., Attalah, S. A., Absy G. M. & Doaa, H. K. (2015). Effect of plant extract of Tribulus terrestris and probiotics on the reproductive performance, total cholesterol and testosterone hormone levels of rams, MENA Science Journal, 1, 14 – 19.
Ștefanescu, R., Tero-Vescan, A., Negroiu, A., Aurica, E. & Vari, C. E. (2020). A comprehensive review of the phytochemical, pharmacological, and toxicological properties of Tribulus terrestris L, Biomolecules, 10(5), 752.
Tomova, M., Gyulemetova, V. & Zarkova, S. (1978). Author's certificate, N 77584 A 61 K 35/1978 (BG).
Tomova, M. & Panova, D. (1965). Steroid sapogenins. Isolation of diosgenin from Tribulus terrestris L. Farmatsiya, 15, 211 - 214 (BG).
Tomova, M., Panova, D., Zarkova, S. & Dikova, V. (1966). Bulgarian Patent. 11:11450. 30 1/02 A61k/1966 (BG).
Tomova, M. P. & Gyulemetova, R. (1978). Steroid saponins and steroid sapogenins. VI. Furostanol bisglycoside from Tribulus terrestris L., Planta Medica, 34, 188 - 191 (BG).
Vahedi, V., Hedayat Evrigh, N., Behroozlak, M. & Dirandeh, E. (2018). Antioxidant Effects of Thyme (Thymus vulgaris) Extract on Ram Sperm Quality During Cryopreservation. Iranian Journal of Applied Animal Science, 8(2), 263 – 269.
Vineetha (2014). 22 Amazing benefits and uses of Thyme. Health Beckon. 2014. http://www.healthbeckon.com/thyme-benefits/.
Yousef, M. I. (2005). Reproductive performance, blood testosterone, lipid peroxidation and seminal plasma biochemistry of rabbits as affected by feeding Acacia saligna under subtropical conditions, Food Chem. Toxicol., 43, 333 – 339.
Zarhouti, A., Mbaye, M. M., Addoum, B., Louanjli, N., El Khalfi, B. & Soukri, A. (2023). The impact of Origanum vulgare supplementation on human asthenozoospermic sperm parameter quality. The Scientific World Journal, Article ID 8093795, 6. |
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| Date published: 2025-06-25
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