In ovo induced hepatic preneoplasia by n-nitrosodimethylamine and n-nitrosodiethylamine in guinea fowl embryos
Branimir Nikolov
Abstract: Avian embryos have been gaining increasing scientific interest as a valuable model system for the experimental cancer research, that could contribute to a significant reduction of the number of laboratory animals. The toxic and carcinogenic effects induced in ovo by N-nitrosodimethylamine (NDMA) and N-nitrosodiethylamine (NDEA) in guinea fowl embryos have been examined by means of pathoanatomical and histopathological methods. The obtained results indicate that both compounds induce preneoplastic hepatic alterations. The spectrum of macroscopic and microscopic lesions identified in carcinogen treated embryos has been presented and the potential use of avian embryos as an inexpensive and reliable model system for studies on the hepatocarcinogenesis has been discussed.
Keywords: avian embryos; guinea fowl; hepatic preneoplasia; in ovo tests; N-nitrosodiethylamine; N-nitrosodimethylamine
Citation: Nikolov, B. (2025). In ovo induced hepatic preneoplasia by n-nitrosodimethylamine and n-nitrosodiethylamine in guinea fowl embryos. Bulgarian Journal of Animal Husbandry, 62(2), 51-56.
| References: (click to open/close) | Anadón, A., Martinez, M., Castellano, V. & Martinez-Larranaga, M. (2014). The role of in vitro methods as alternatives to animals in toxicity testing. Expert opinion on drug metabolism & toxicology, 10(1), 67-79.
Bannasch, P., Haertel, T. & Su, Q. (2003). Significance of hepatic preneoplasia in risk identification and early detection of neoplasia. Toxicol Pathol, 31, 134-139.
Benigni, R., Bossa, C. & Tcheremenskaia, O. (2013). Improving carcinogenicity assessment. Mutagenesis, 28, 107-116.
Enzmann, H. & Brunnemann, K. (1997). The in ovo carcinogenicity assay (IOCA): A review of an experimental approach for research on carcinogenesis and carcinogenicity testing. Front Biosci, 2, 30-39.
Enzmann, H., Brunnemann, K., Iatropoulos, M., Shpyleva, S., Lukyanova, N., Todor, I., Moored, M., Spichera, K., Chekhunc, V., Tsudad, H. & Williams, G. (2013). Inter-laboratory comparison of turkey in ovo carcinogenicity assessment (IOCA) of hepatocarcinogens. Exp. Toxicol. Pathol., 65, 729-735.
Enzmann, H., Brunnemann, K. D., Iatropoulos, M. & Williams, G. M. (1996). Induction of hyperplastic lesions in embryonic quail liver in ovo. Proc. AACR Annual Meeting. Abstr. 777, Washington, DC, April, 20-24.
Enzmann, H. G., Kaliner, B., Watta-Gebert & Löser, E. (1992). Foci of altered hepatocytes induced in embryonal turkey liver. Carcinogenesis, 13, 943-946.
Enzmann, H.G., Kuhlem, C., Loser, E. & Bannasch, P. (1995b). Damage to mitochondrial DNA induced by the hepatocarcinogen diethylnitrosamine in ovo. Mutat. Res., 329(2), 113-120.
Enzmann, H. G., Kuhlem, C., Kaliner, G., Loser, E. & Bannasch, P. (1995a). Rapid induction of preneoplastic liver foci in embryonal turkey liver by diethylnitrosamine. Toxicologic pathology, 23(5), 560-569.
Knight, A., Bailey, J. & Balcombe, J. (2006). Animal carcinogenicity studies: implications for the REACH system. Altern. Lab. Anim., 34, 139-147.
Marone, P., Hall, W. & Hayes, A. (2014). Reassessing the two-year rodent carcinogenicity bioassay: a review of the applicability to human risk and current perspectives. Regul. Toxicol. Pharm., 68, 108-118.
Nikolov, B. (2024). N-nitrosodimethylamine and n-nitrosodiethylamine induced hepatic preneoplasia in turkey embryos. Tradition and modernity in veterinary medicine, 9(2), 15-20.
Nikolov, B., Georgieva, A., Pepovich, R., Hristov, K., Mehmedov, T., Manov, V., Nikolova, E., Petrova, R., Vladov, I. & Kril, A. (2016). Hepatic preneoplasia induced by n-nitrosodimethylamine and n-nitrosodiethylamine in japanese quail embryos. Tradition and modernity in veterinary medicine, 1(1), 21-25.
Pitot, H. (2007). Adventures in hepatocarcinogenesis. Annu. Rev. Pathol., 2, 1-29.
Tsuda, H., Futakuchi, M., Fukamachi, K., Shirai, T., Imaida, K., Fukushima, S., Tatematsu, M., Furukawa, F., Tamano, S. & Ito, N. (2010). A medium-term, rapid rat bioassay model for the detection of carcinogenic potential of chemicals. Toxicol. Pathol., 38, 182-187.
Williams, G. M., Brunnemann, K. D., Iatraopoulos, M. J., Smart, D. J. & Enzmann, H. G. (2011). Production of liver preneoplasia & gallbladder agenesis in turkey fetuses administered diethylnitrosamine. Arch. Toxicol., 85, 681-687. |
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| Date published: 2025-04-29
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