PCR-RFLP assay for genotyping of three different regions of the BMP15 gene in sheep from the Bulgarian dairy synthetic population

Milena Bozhilova-Sakova, Ivona Dimitrova, Nevyana Stancheva, Radena Nenova, Todor Tzonev, Maya Ignatova

Abstract: Bone morphogenetic protein 15 (BMP15/FecX) gene is one of the candidate genes for the reproduction in farm animals, especially sheep. The present study aimed to detect the genetic polymorphism in three different regions of BMP15 gene in sheep from the Bulgarian Dairy Synthetic Population (BDSP) using PCR-RFLP technique. For the purpose of the experiment were used 163 ewes from the herd of
the Agricultural Institute-Shumen with records of the number of lambs born from a minimum of two consecutive lambing. The average number lambing of ewe is 4.07. DNA was extracted from blood samples of all ewes, subjected to PCR amplification. The PCR products of 141 bp (FecXG), 153 bp (FecXB), and 240 bp (FecXH) were cut with HinƒI, DdeI, and SpeI restriction enzymes, respectively.
In FecXG region were visualized two fragments with length 112 bp and 29 bp (wild genotype ++) in all tested ewes. For FecXB in all animals were obtained two fragments of 122 bp and 31 bp which corresponded to homozygous wild genotype AA. For FecXH all animals were homozygous with CC genotype and only one determined fragment of 240 bp. In conclusion, the findings in present study did not detect any polymorphisms in BMP-15 gene in sheep, regardless their good prolificacy.

Keywords: BMP15 gene; Bulgarian Dairy Synthetic Population; genetic polymorphism; PCR-RFLP; Sheep

Date published: 2023-04-28