EFFECT OF PLANT EXTRACTS WITH ANTIVIRAL PROPERTIES ON THE SURVIVAL RATE OF PIG OOCYTES
Mihail Chervenkov, Teodora Ivanova, Dimitrina Kacheva, Tatiana Stoeva, Elena Kistanova
Резюме: The use of mechanical methods for disinfection of oocytes and embryos alone or in combination with enzymes (mainly trypsin), when performing assisted reproductive technology (ART) in livestock are not effective against all kind of viruses. Therefore, it is essential the search for new antiviral agents,
which have no adverse effect on the oocyte and embryo development. The aim of our study was to determine the optimal, non toxic concentrations of extracts from Melissa officinalis and Leucojum aestivum for porcine oocytes. These are plants with known antiviral properties and widely used in medicine. The oocytes were obtained from clinically healthy pigs, slaughtered in an approved slaughterhouse. Oocytes were treated with plant extracts at dilutions from 1:7 to 1:320 for Melissa officinalis, and from 1:10 to 1:500 for Leucojum aestivum. The assessment of the oocytes survival rate, was made on the base of morphological examination and staining with trypan blue. The dilutions of the extracts, used for oocytes treatment, were defined, after preliminary testing on MDBK cell line. The obtained data shows, that the survival rate of the oocytes, after treatment with extract of M. officinalis, at each tested dilution was above 96.00%. Regarding the treatment with extract of L. aestivum, the percentage of alive oocytes, was more than 88.00%, at each tested dilution. In both cases, the untreated control groups, had survival rate of 100%.
which have no adverse effect on the oocyte and embryo development. The aim of our study was to determine the optimal, non toxic concentrations of extracts from Melissa officinalis and Leucojum aestivum for porcine oocytes. These are plants with known antiviral properties and widely used in medicine. The oocytes were obtained from clinically healthy pigs, slaughtered in an approved slaughterhouse. Oocytes were treated with plant extracts at dilutions from 1:7 to 1:320 for Melissa officinalis, and from 1:10 to 1:500 for Leucojum aestivum. The assessment of the oocytes survival rate, was made on the base of morphological examination and staining with trypan blue. The dilutions of the extracts, used for oocytes treatment, were defined, after preliminary testing on MDBK cell line. The obtained data shows, that the survival rate of the oocytes, after treatment with extract of M. officinalis, at each tested dilution was above 96.00%. Regarding the treatment with extract of L. aestivum, the percentage of alive oocytes, was more than 88.00%, at each tested dilution. In both cases, the untreated control groups, had survival rate of 100%.
Ключови думи: antiviral; Leucojum aestivum; Melissa officinalis; oocytes; pig; sanitary
Дата на публикуване: 2023-01-31
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