INVESTIGATIONS THE EFFECTS OF AFLATOXIN B1 ON RENAL FUNCTION IN MULARD DUCKS
I. Valchev, N. Grozeva, L. Lazarov, D. Kanakov, Ts. Hristov, Y. Nikolov
Резюме: The toxic effect of AFB1 in mulard ducks was evaluated through the changes in several blood parameters, namely uric acid, urea, creatinine, total protein, calcium, inorganic phosphate, sodium and potassium. Also, the morphological changes in kidney parenchyma were evaluated. Experiments were conducted with 4 groups of 20 10-day-old mulard ducks each.
The groups were as followed: group I – control, fed a standard compound feed according to the species and the age; group II – experimental, whose feed was supplemented with 0.5 mg/kg AFB1, group III – experimental, supplemented with 0.8 mg/kg AFB1 and group IV – experimental, supplemented with 0.5 mg/kg AFB1 and 2 g/kg Mycotox NG. The duration of the experiment was 42 days. The blood analysis results by the 21st day of the trial showed reduced concentrations of uric acid, urea, creatinine, total protein, calcium, inorganic phosphate, sodium and potassium in groups II and III. These changes
tended to become more pronounced by the 42nd day from the beginning of the experiment. Morphological alterations of kidney parenchyma consisted in degeneration in renal tubules, congestion, desquamation and disintegration of tubular cells with pyknotic changes in cell nuclei, necrobiotic changes and haemorrhages depending on the amount of ingested toxin.
The dietary supplementation of group IV with Mycotox NG decreased the severity of observed blood changes as well as the frequency and severity of histological lesions (hyperaemia and mild granular dystrophy with pyknotic nuclei).
The groups were as followed: group I – control, fed a standard compound feed according to the species and the age; group II – experimental, whose feed was supplemented with 0.5 mg/kg AFB1, group III – experimental, supplemented with 0.8 mg/kg AFB1 and group IV – experimental, supplemented with 0.5 mg/kg AFB1 and 2 g/kg Mycotox NG. The duration of the experiment was 42 days. The blood analysis results by the 21st day of the trial showed reduced concentrations of uric acid, urea, creatinine, total protein, calcium, inorganic phosphate, sodium and potassium in groups II and III. These changes
tended to become more pronounced by the 42nd day from the beginning of the experiment. Morphological alterations of kidney parenchyma consisted in degeneration in renal tubules, congestion, desquamation and disintegration of tubular cells with pyknotic changes in cell nuclei, necrobiotic changes and haemorrhages depending on the amount of ingested toxin.
The dietary supplementation of group IV with Mycotox NG decreased the severity of observed blood changes as well as the frequency and severity of histological lesions (hyperaemia and mild granular dystrophy with pyknotic nuclei).
Ключови думи: Aflatoxin B1, uric acid; calcium; creatinine; inorganic phosphate; mallard ducklings; morphological changes in kidney; potassium; sodium; total protein; urea
Дата на публикуване: 2017-03-20
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