EFFECT OF VITAMIN E AND CYSTEINE ON CRYOPRESERVED BULL SEMEN

T. Todorova

Резюме: Aim of our study was to test the hypothesis that the different levels of vitamin E and cysteine can protect effectively bull sperm against oxidative damage during cryopreservation in various diluents and lead to greater viability and motility of the sperm after thawing.
The results show that the lowest amount of malondialdehyde (MDA), as an indicator of lipid peroxi-dation (CLP) was observed with the addition of 5 mM cysteine to citrate sperm extender.
The lowest value of malondialdehyde (MDA) as an indicator of lipid peroxidation (CLP) was ob-served after adding 0.1 mM vitamin E to citrate sperm extender.
Based on these results, we reach the conclusion that cysteine and vitamin E are very effective anti-oxidants in citrate sperm extender (CR) and that their addition to such one can reduce oxidative stress caused by thawing and protects bovine sperm by cryopreservation. The addition of cysteine and vitamin E to Tris - egg yolk sperm extender (TP) does not improve the quality of the semen. The results of this study provide a new approach to cryopreservation of semen from a bull, and can help to improve the results of cryopreservation of semen in industrial farming.

Ключови думи: bull; cysteine; vitamin E

Дата на публикуване: 2017-03-16