The use of biotechnologica l methods for cons ervat ion of an imal genetic resources

SVITLANA KOVTUN, OKSANA SHCHERBAK, AZA ZYUZYUN, NATALIIA RIEZNYKOVA, ALEXANDER DUVANOV,, ALEXANDER OSYPCHUK

Abstract: In the research there were proved the efficiency of modern biotechnological methods application for the conservation of gene pool and improving of the profitability of cattle-breeding. There were combined the sexed sperm artificial insemination method with transfer of Holstein bovine embryos in “Agro-Soyuz” cattle farm. It was found that embryo forming level after using of sexed semen for insemination of donor cows did not differ significantly compared with the use of non-sexed semen. But at using of sexed semen for insemination of cows there were detected much lower level (P <0.05, the criterion χ2) of suitable for embryo transplantation embryo forming (55.6%, 10 of 18 embryos) when compared with non-sexed semen (87.5 %, 23 of 32 embryos). For the last years we got and transplanted 211 sexed embryos at the farm “Agro-Soyuz”. Our DNA research of Holstein degenerated embryos, using PCR-analysis, showed that all researched embryos were female’s. Embryo transfer was used at small Grey Ukrainian cattle keeping farm for more effective expanded use of valuable adaptive genetic potential of this breed, animals of which are used longer and are healthier, than other breeds.

Keywords: biotechnological methods; bovine embryo transfer; genetic resources; sexed semen of bulls

Date published: 2023-01-31