INTRACYTOPLASMATICAL INJECTIONS USING EJACULATED BOAR SPERM

Iancho Todorov, Tania Todorova

Abstract: The study was to test the effect of the injection of ejaculated boar sperm in in vitro maturated oocytes and determine the fertilization and development rates. Experiments were performed with the use of in vitro maturated oocytes and ejaculated and diluted sperm. Controls groups were injected with diluent and non-injected oocytes. After culturing in FTM (Fert Talp Medium) for 20 hours, the injected oocytes and controls were stained and analyzed for the development of the pronuclei. Fertilized eggs were cultured in NCSU 23 for 48 hours. Evolution was noted at 48 hours. From the injected oocytes 43.6% were fertilized and began dividing. Only 4. 19% continued development over 4 blastomeres. The results were encouraging, but require more rigorous testing, particularly for pigs in which the data from the application of the ICSI is relatively small as compared to other species, especially in man.

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Date published: 2023-01-31