Influence of the transportation of cow ovaries on the IVM/IVF/IVC results: A report
Tsvetelina Todorova, Iancho Todorov
Abstract: Ovaries from 12 cows were collected separately in an abattoir and the data concerning the donor
were recorded. The ovaries were transported for 8–10 hours to the laboratory and processed immediately.
Oocytes from these ovaries were collected separately and submitted to IVM/IVF/IVC. The
seven HF cows supplied 165 oocytes (23.57 per cow on average) with high individual variations. Five
MB cows supplied 250 oocytes (50 per cow on average). After IVF 137 expanded blastocysts were
obtained (33% of oocytes, 11.41 per cow, range 2–22). All of the obtained blastocysts were frozen by
vitrification.
were recorded. The ovaries were transported for 8–10 hours to the laboratory and processed immediately.
Oocytes from these ovaries were collected separately and submitted to IVM/IVF/IVC. The
seven HF cows supplied 165 oocytes (23.57 per cow on average) with high individual variations. Five
MB cows supplied 250 oocytes (50 per cow on average). After IVF 137 expanded blastocysts were
obtained (33% of oocytes, 11.41 per cow, range 2–22). All of the obtained blastocysts were frozen by
vitrification.
Keywords: bovine; culture; in vitro; individual cow
Date published: 2022-04-26
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