DIFFERENTIATION OF TYPES MYCOBACTERIA FROM MYCOBACTERIUM AVIUM COMPLEX

T. Savova, M. Bonovska, A. Dimitrova, Nikolay Lalkovski

Abstract: Mycobacterium avium complex (MAC) includes M. avium, M. paratuberculosis, M. lepraemurium and M. intracellulare. Mycobacterium avium subsp. avium (MAA) and M. avium subsp. рaratuberculosis (MAР) are pathogenic bacteria with a high degree of identity to the nucleotide sequences in certain regions of their genome. There has been a comparative study of specificity of strains of Mycobacterium avium subsp. avium (MAA) and M. avium subsp. paratuberculosis (MAP) by bacteriological (solid media Herrold`s Egg and Löwenstein-Jensen and liquid media) and the molecular biological methods (conventional PCR) with two commercial diagnostic kits (respectively specific for M. avium and M. paratuberculosis). It has been found that commercial PCR kits does not differentiate two test species of mycobacteria, as opposed to the microbiological method by isolation of solid media.

Keywords: differentiation; microbiology; Mycobacterium avium complex (MAC); PCR

Date published: 2017-11-10