In vitro cultivation of bovine embryos
Tsvetelina Todorova
Abstract: Embryo cultivation is the longest step during the in vitro embryo production process. In this study, the effect of adding 5% FCS or 3 mg/ml BSA to SOF and KSOM in vitro culture media was investigated. When adding FCS in SOF medium, a better effect on separation was observed (P < 0.05), and the percentage of blastocysts was higher - 58.6%. When BSA was added to SOF medium, the percentage of blastocysts was 40.1% - significantly lower. When cultured in KSOM medium with the addition of FCS, the division rate was lower than when cultured in SOF+FCS and the proportion of blastocysts was also slightly lower. When BSA was added to the KSOM medium, the percentage of blastocysts was almost as in the SOF+BSA group. Аt the conclusion of the conducted experiment, the addition of FCS in KSOM and SOF media is beneficial for in vitro cultivation, resulting in a higher percentage of blastocysts.
Keywords: bovine; BSA; embryos; FCS; KSOM; SOF
Citation: Todorova, Ts. (2024). In vitro cultivation of bovine embryos. Bulgarian Journal of Animal Husbandry, 61(5), 25-29 (Bg).
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| Date published: 2024-10-24
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