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Bulgarian Jurnal of Animal Husbandry   ISSN 0514-7441
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Effects of Zoovit probiotic on faecal microbiota in cattle
Georgi Chavdarov, Nikolay Ivanovorcid, Ivan Slavovorcid, Stayka Lalevaorcid
Abstract: The amount of pathogenic bacteria in feces is an indicator characterizing the health status of animals. This is important to protect the environment and the health of farm workers.
The aim of the present study is to determine the effect of probiotic Zoovit on the microbiome of the faecal mass of Holstein cattle.
In the experiment, two groups were formed - experimental and control, 30 each. A probiotic was added to the combined feed of the experimental group, but not to the control group. The food ration with the participation of probiotic is placed twice a day.
To determine the microbiome in fecal matter in cattle, PCR analyzes were used for the detection of Lactobacillus delbrueckii subsp. bulgaricus, a species-specific PCR assay for the presence of total DNA in L. delbrueckii and L. delbrueckii subsp. bulgaricus and methods for determination of sulfite-reducing colonies.
No representatives of Staphulococccua aureus and Clostridium sp were isolated in the faecal mass of the animals from both groups. For Escherichia coli and Coliforms, a significant decrease in the faecal mass of cows fed with feed containing probiotics was found.
The presence of L. delbrueckii subsp. bulgaricus in animals receiving a probiotic. Colonies with a colonial characteristic typical of L. delbrueckii subsp. bulgaricus were isolated in the animals receiving probiotic Zoovit and in the control group, no colonies typical of Lactobacillus bulgaricus were found.
Keywords: faecal microbiota; Holstein; PCR amplification; PCR analysis; probiotics
Citation: Chavdarov, G., Ivanov, N., Slavov, I. & Laleva, S. (2024). Effects of Zoovit probiotic on faecal microbiota in cattle. Bulgarian Journal of Animal Husbandry, 61(1), 11-18.
References: (click to open/close)
DOI: https://doi.org/10.61308/OCYQ3517
Date published: 2024-02-26
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