Microsatellite DNA profile in the East Bulgarian horse breed

Iliyana Sabeva

Резюме: The microsatellite DNA markers and the genetic diversity in East Bulgarian horses was studied.
Subject of the analyzes were data of nucleotide polymorphism (SSRs) in 15 loci of 403 horses born
after 2010 and registered in the East Bulgarian Horse Association.
The highest polymorphism was established in ASB23, ASB2, ASB17 and VHL20 loci, while HMS1,
HTG4 and HTG7 were with the lowest. The effective number of alleles at all loci in the conditions of
moderate genetic diversity in the population was approximately two times lower than observed. To
the “rare” allele category, the allele B in the ASB2 locus, allele U in ASB23, allele F in ASB17, allele
S in ASB23 and HTG10, allele T in ASB23 and HMS3 can be included. All loci contained the M and
N alleles. Only ASB23 and HMS1 lack the O allele.
Increased diversity was observed in the HTG10, ASB17, ASB23, ASB2, HMS2, HMS3, and HMS7
loci with respect to Ho and He. Excess of heterozygotes was observed due to the widespread use of
crossbreeding during the last two generational intervals. The intra-population inbreeding coefficient
and the average inter-locus correlation were low in values. Statistically proven interactions of linkage
disequilibrium (LD) were found for 93 allelic combinations of the studied loci.
The microsatellite DNA profile of the East Bulgarian breed is close to those of the previous two
generations of English Thoroughbreds developed in Bulgaria.

Ключови думи: East Bulgarian riding horse; genetic diversity; microsatellite markers

Дата на публикуване: 2021-08-27