MORPHOMETRIC PARAMETERS OF OVARIAN STRUCTURES IN RABBITS, FED WITH ADDITIVE TRIBULUS TERRESTRIS L. EXTRACT
D. Abadjieva, Sv. Grigorova, G. Vulchev, E. Kistanova
Резюме: The aim of this study was the determination of morphometric parameters of ovarian structures (follicles and oocytes) in rabbits treated with Bulgarian product VemoHerb-T (dry extract from Tribulus terrestris L.).
A total of 45 New Zealand White female growing rabbits were divided into three groups: control, I-st experimental group was treated with 2.5 mg/kg of Tribulus t. extract and II-nd experimental group - with 10 mg/kg of TT extract by drinking water, once daily for 42 days. After slaughter of animals, fixed in formalin ovaries were embedded in paraffin to obtain se¬rial sections for light microscopy and developed by standard histological methods. The following morphometric parameters were examined: diameter (μm) of the follicles and oocytes, and the ratio between volume of granulose cells and oocytes (%) in primary, secondary and preovulatory follicles.
The results showed that the dose 2.5 mg / kg body weight VemoHerb-T did not contribute to morphometric structural changes and did not disturb the normal folliclegenesis. The addition of 10 mg / kg body weight Tribulus terrestris L. extract lead to increasing the size of the follicles and oocytes in the rabbit ovarian since the stage of secondary follicles. However, in the preovulatory follicles of these experimental animals degenerative changes of granulose cells, leading to deterioration of oocytes quality and follicular atresia, were observed.
A total of 45 New Zealand White female growing rabbits were divided into three groups: control, I-st experimental group was treated with 2.5 mg/kg of Tribulus t. extract and II-nd experimental group - with 10 mg/kg of TT extract by drinking water, once daily for 42 days. After slaughter of animals, fixed in formalin ovaries were embedded in paraffin to obtain se¬rial sections for light microscopy and developed by standard histological methods. The following morphometric parameters were examined: diameter (μm) of the follicles and oocytes, and the ratio between volume of granulose cells and oocytes (%) in primary, secondary and preovulatory follicles.
The results showed that the dose 2.5 mg / kg body weight VemoHerb-T did not contribute to morphometric structural changes and did not disturb the normal folliclegenesis. The addition of 10 mg / kg body weight Tribulus terrestris L. extract lead to increasing the size of the follicles and oocytes in the rabbit ovarian since the stage of secondary follicles. However, in the preovulatory follicles of these experimental animals degenerative changes of granulose cells, leading to deterioration of oocytes quality and follicular atresia, were observed.
Ключови думи: follicle; oocyte; rabbits; Tribulus terrestris
Дата на публикуване: 2017-05-23
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