Effects of Zoovit probiotic on faecal microbiota in cattle

Georgi Chavdarov; Nikolay Ivanov; Ivan Slavov; Stayka Laleva

bg | en

Georgi Chavdarov, Nikolay Ivanov

, Ivan Slavov

, Stayka Laleva

Резюме: The amount of pathogenic bacteria in feces is an indicator characterizing the health status of animals. This is important to protect the environment and the health of farm workers.
The aim of the present study is to determine the effect of probiotic Zoovit on the microbiome of the faecal mass of Holstein cattle.
In the experiment, two groups were formed - experimental and control, 30 each. A probiotic was added to the combined feed of the experimental group, but not to the control group. The food ration with the participation of probiotic is placed twice a day.
To determine the microbiome in fecal matter in cattle, PCR analyzes were used for the detection of Lactobacillus delbrueckii subsp. bulgaricus, a species-specific PCR assay for the presence of total DNA in L. delbrueckii and L. delbrueckii subsp. bulgaricus and methods for determination of sulfite-reducing colonies.
No representatives of Staphulococccua aureus and Clostridium sp were isolated in the faecal mass of the animals from both groups. For Escherichia coli and Coliforms, a significant decrease in the faecal mass of cows fed with feed containing probiotics was found.
The presence of L. delbrueckii subsp. bulgaricus in animals receiving a probiotic. Colonies with a colonial characteristic typical of L. delbrueckii subsp. bulgaricus were isolated in the animals receiving probiotic Zoovit and in the control group, no colonies typical of Lactobacillus bulgaricus were found.

Ключови думи: faecal microbiota; Holstein; PCR amplification; PCR analysis; probiotics

Цитиране: Chavdarov, G., Ivanov, N., Slavov, I. & Laleva, S. (2024). Effects of Zoovit probiotic on faecal microbiota in cattle. Bulgarian Journal of Animal Husbandry, 61(1), 11-18.

Литература: (click to open/close)

Anne, I., Alam, S., Begum, R., Shahjahan, R. & Khandaker, M. A. (2021). The role of probiotics on animal health and nutrition. The Journal of Basic and Applied Zoology, 82(52), 2-16.
Elshaghabee, F. M. F. & Rokana, N. (2022). Mitigation of antibiotic resistance using probiotics, prebiotics and synbiotics. A review. Environmental Chemistry Letters, 20, 1295–1308.
Ghazanfar, S., Anjum, I. M., Azim, A. & Ahmed, I. (2015). Effects of dietary supplementation of yeast (Saccharomyces cerevisiae) culture on growth performance, blood parameters, nutrient digestibility and fecal flora of dairy heifers. The Journal of Animal & Plant Sciences, 25(1), 53-59.
Ghazanfar, S., Qubtia, M., Ahmed, I., Hasan, F., Anjum, I. M. & Imran, M. (2018). Effect of indigenously isolated saccharomyces cerevisiae Probiotics on milk production, nutrient digestibility, blood Chemistry and fecal microbiota in lactating dairy cows. The Journal of Animal and Plant Sciences, 28(2), 407-420.
Guo, Y., Li, Z., Deng, M., Li, Y., Liu, G., Liu, D., Lui, Q., Lui, Q. & Sun, B. (2022). Effects of a multi-strain probiotic on growth, health, and fecal bacterial flora of neonatal dairy calves. Animal Bioscience, 35(2), 204-216.
Jatkauskas, J. & Vrotniakiene. V. (2010). Effects of probiotic dietary supplementation on diarrhea patterns, faecal microbiota and performance of early weaned calves. Veterinarna. Medicina, 55(10), 494-503.
Kabir, E., Alam, J., Hossain, M. & Ferdaushi, Z. (2022). Effect of feeding probiotic fermented rice straw-based total mixed ration on production, blood parameters and faecal microbiota of fattening cattle. Journal of Animal Health and Production, 10(2), 190-197.
Kawakami, S., Yamada, T., Nakanishi, N. & Cai, Y. (2010). Feeding of lactic acid bacteria and yeast on growth and diarrhea of Holstein calves. Journal of Animal and Veterinary Advances, 9(7), 1112-1114.
Lick, S., Brockmann, E. & Heller, J. K. (2000). Identification of Lactobacillus delbrueckii and Subspecies by Hybridization Probes and PCR. Systematic and Applied Microbiology, 23(2), 251-259.
Lick, S., Drescher, K. & Heller, J. K. (2001). Survival of Lactobacillus delbrueckii subsp. bulgaricus and Streptococcus thermophilus in the Terminal Ileum of Fistulated Göttingen Minipigs. Applied and Environmental Microbiology, 67(9), 4137–4143.
Mansilla, I. F., Miranda, H. M., Uezen, D. J., Maldonado, C. N., Villar, D. M., Merino, A. L., Vignolo, M. G. & Nader-Macias, F. E. M. (2023). Effect of probiotic lactobacilli supplementation on growth parameters, blood profile, productive performance, and fecal microbiology in feedlot cattle. Veterinary Science, 155(6), 76-87.
Razzaque, S. M. (2021). Commentary: microbial resistance movements: an overview of global public health threats posed by antimicrobial resistance, and how best to counter. Frontiers in Public Health, 8, 1-4, 535668.
Tufarelli, V., Crovace, M. A., Rossi, G. & Laudadio, V. (2017). Еffect of a dietary probiotic blend on performance, blood characteristics, meat quality and faecal microbial shedding in growing-finishing pigs. South African Journal of Animal Science, 47(6), 875-882.
Vadopalas, L., Zokaityte, E., Zavistanaviciute, P., Gruzauskas, R., Starkute, V., Mockus, E., Klementaviciute, J., Ruzauskas, M., Lele, V., Cernauskas, D., Klupsaite, D., Dauksiene, A., Sederevicius, A. Badaras, E. & Bartkiene, E. (2021). Supplement Based on Fermented Milk Permeate for Feeding Newborn Calves: Influence on Blood, Growth Performance, and Faecal Parameters, including Microbiota, Volatile Compounds, and Fatty and Organic Acid Profiles. Animals, 11, 2-15, 2544.
Xu, H., Huang, W., Hou, Q., Kwok, L., Sun, Z., Ma, H., Zhao, F., Lee, Y. & Zhang, H. (2017). The effects of probiotics administration on the milk production, milk components and fecal bacteria microbiota of dairy cows. Science Bulletin, 62, 11, 767-774.
Zalewska, M., Błażejewska, A., Czapko, A. & Popowska, M. (2021). Antibiotics and antibiotic resistance genes in animal manure – consequences of its application in agriculture. Front. Microbiol. 12, 1-21, 610656, doi:10.3389/fmicb.
Zamojska, D., Nowak, A., Nowak, I. & Macierzyńska-Piotrowska, E. (2021). Probiotics and Postbiotics as Substitutes of Antibiotics in Farm Animals: A Review. Animals, 11(12), 2-20, 3431.
Zhang, Z., Yang, L., He, Y., Luo, X., Zhao, S, & Jia, X. (2021). Composition of Fecal microbiota in grazing and feedlot angus beef cattle. Animals, 11(11), 2-8,3167.

DOI: https://doi.org/10.61308/OCYQ3517

Дата на публикуване: 2024-02-26

Свали пълен текст